Rapid solid-phase radioimmunoassay for staphylococcal enterotoxin A.
نویسندگان
چکیده
A rapid solid-phase radioimmunoassay for staphylococcal enterotoxin A is described. The assay procedure requires 3 to 4 h for completion by using a competitive inhibition system in which the antibody is attached to bromacetyl cellulose particles. It is accurate to a level of 0.01 mug of enterotoxin A/ml in a variety of media such as ham, milk products, crab meat, custard, etc. No significant interference was found with any media or food product tested.
منابع مشابه
Staphylococcal enterotoxins A and B: solid-phase radioimmunoassay in food.
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A sensitive radioimmunoassay utilizing Staphylococcus aureus cells containing protein A as a coprecipitant was developed for the detection and quantitation of staphylococcal enterotoxins A, B, C, D, and E in a variety of foods. The enterotoxins were extracted from the foods by a simple and rapid procedure. The sensitivity of the assay is 1.0 ng or less of enterotoxin per g of food.
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ورودعنوان ژورنال:
- Applied microbiology
دوره 25 5 شماره
صفحات -
تاریخ انتشار 1973